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SHIMIZU Tatsuya
Department Research Institutes and Facilities, Research Institutes and Facilities Position Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets. |
| Journal | Formal name：Macromolecular bioscience Abbreviation：Macromol Biosci ISSN code：(1616-5195)1616-5187(Linking) |
| Domestic / Foregin | Foregin |
| Publisher | Wiley |
| Volume, Issue, Page | 17(8),pp.1700073 |
| Author and coauthor | Nagase Kenichi†, Nagumo Yuhei, Kim Miri, Kim Hee-Jung, Kyung Hei-Won, Chung Hye-Jin, Sekine Hidekazu, Shimizu Tatsuya, Kanazawa Hideko, Okano Teruo, Lee Seung-Jin, Yamato Masayuki* |
| Publication date | 2017/05 |
| Summary | Cell sheet transplantation is a key tissue engineering technology. A vascular endothelial growth factor (VEGF)-releasing fiber mat is developed for the transplantation of multilayered cardiomyocyte sheets. Poly(vinyl alcohol) fiber mats bearing poly(lactic-co-glycolic acid) nanoparticles that incorporate VEGF are fabricated using electrospinning and electrospray methods. Six-layered cardiomyocyte sheets are transplanted with a VEGF-releasing mat into athymic rats. After two weeks, these sheets produce thicker cardiomyocyte layers compared with controls lacking a VEGF-releasing mat, and incorporate larger-diameter blood vessels containing erythrocytes. Thus, local VEGF release near the transplanted cardiomyocytes induces vascularization, which supplies sufficient oxygen and nutrients to prevent necrosis. In contrast, cardiomyocyte sheets without a VEGF-releasing mat do not survive in vivo, probably undergo necrosis, and are reduced in thickness. Hence, these VEGF-releasing mats enable the transplantation of multilayered cardiomyocyte sheets in a single procedure, and should expand the potential of cell sheet transplantation for therapeutic applications. |
| DOI | 10.1002/mabi.201700073 |
| PMID | 28547766 |