シミズ タツヤ   SHIMIZU Tatsuya
  清水 達也
   所属   研究施設 研究施設
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Controlling shape and position of vascular formation in engineered tissues by arbitrary assembly of endothelial cells
掲載誌名 正式名:Biofabrication
略  称:Biofabrication
ISSNコード:(1758-5090)1758-5082(Linking)
掲載区分国外
巻・号・頁 7(4),045006頁
著者・共著者 Takehara Hiroaki†, Sakaguchi Katsuhisa, Kuroda Masatoshi, Muraoka Megumi, Itoga Kazuyoshi, Okano Teruo, Shimizu Tatsuya*
発行年月 2015/11
概要 Cellular self-assembly based on cell-to-cell communication is a well-known tissue organizing process in living bodies. Hence, integrating cellular self-assembly processes into tissue engineering is a promising approach to fabricate well-organized functional tissues. In this research, we investigated the capability of endothelial cells (ECs) to control shape and position of vascular formation using arbitral-assembling techniques in three-dimensional engineered tissues. To quantify the degree of migration of ECs in endothelial network formation, image correlation analysis was conducted. Positive correlation between the original positions of arbitrarily assembled ECs and the positions of formed endothelial networks indicated the potential for controlling shape and position of vascular formations in engineered tissues. To demonstrate the feasibility of controlling vascular formations, engineered tissues with vascular networks in triangle and circle patterns were made. The technique reported here employs cellular self-assembly for tissue engineering and is expected to provide fundamental beneficial methods to supply various functional tissues for drug screening and regenerative medicine.
DOI 10.1088/1758-5090/7/4/045006
PMID 26545138