Akitsugu Kawashima
   Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine
   Position   Associate Professor
Article types Original article
Language English
Peer review Peer reviewed
Title RNA sequencing analysis revealed the induction of CCL3 expression in human intracranial aneurysms.
Journal Formal name：Scientific reports
Abbreviation：Sci Rep
ISSN code：20452322/20452322
Domestic / ForeginForegin
Volume, Issue, Page 9(1),pp.10387
Author and coauthor Aoki Tomohiro, Koseki Hirokazu, Miyata Haruka, Itoh Masayoshi, Kawaji Hideya, Takizawa Katsumi, Kawashima Akitsugu, Ujiie Hiroshi, Higa Takashi, Minamimura Kenzo, Kimura Toshikazu, Kasuya Hidetoshi, Nozaki Kazuhiko, Morita Akio, Sano Hirotoshi, Narumiya Shuh
Publication date 2019/07
Summary Intracranial aneurysm (IA) is a socially important disease as a major cause of subarachnoid hemorrhage. Recent experimental studies mainly using animal models have revealed a crucial role of macrophage-mediated chronic inflammatory responses in its pathogenesis. However, as findings from comprehensive analysis of unruptured human IAs are limited, factors regulating progression and rupture of IAs in humans remain unclear. Using surgically dissected human unruptured IA lesions and control arterial walls, gene expression profiles were obtained by RNA sequence analysis. RNA sequencing analysis was done with read count about 60~100 million which yielded 6~10 billion bases per sample. 79 over-expressed and 329 under-expressed genes in IA lesions were identified. Through Gene Ontology analysis, 'chemokine activity', 'defense response' and 'extracellular region' were picked up as over-represented terms which included CCL3 and CCL4 in common. Among these genes, quantitative RT-PCR analysis using another set of samples reproduced the above result. Finally, increase of CCL3 protein compared with that in control arterial walls was clarified in IA lesions. Findings of the present study again highlight importance of macrophage recruitment via CCL3 in the pathogenesis of IA progression.
DOI 10.1038/s41598-019-46886-2
PMID 31316152