OKANO Teruo
   Department   Research Institutes and Facilities, Research Institutes and Facilities
   Position  
Article types Original article
Language English
Peer review Peer reviewed
Title Safety and efficacy of human juvenile chondrocyte-derived cell sheets for osteochondral defect treatment.
Journal Formal name：NPJ Regenerative medicine
Abbreviation：NPJ Regen Med
ISSN code：20573995/20573995
Domestic / ForeginForegin
Volume, Issue, Page 6(1),pp.65
Author and coauthor KONDO Makoto†*, KAMEISHI Sumako, KIM Kyungsook, METZLER Nicolas F, MAAK Travis G, HUTCHINSON Douglas T, WANG Angela A, MAEHARA Miki, GRAINGER David W., OKANO Teruo*
Authorship Last author,Corresponding author
Publication date 2021/10/15
Summary Knee cartilage does not regenerate spontaneously after injury, and a gold standard regenerative treatment algorithm has not been established. This study demonstrates preclinical safety and efficacy of scaffold-free, human juvenile cartilage-derived-chondrocyte (JCC) sheets produced from routine surgical discards using thermo-responsive cultureware. JCCs exhibit stable and high growth potential in vitro over passage 10, supporting possibilities for scale-up to mass production for commercialization. JCC sheets contain highly viable, densely packed cells, show no anchorage-independent cell growth, express mesenchymal surface markers, and lack MHC II expression. In nude rat focal osteochondral defect models, stable neocartilage formation was observed at 4 weeks by JCC sheet transplantation without abnormal tissue growth over 24 weeks in contrast to the nontreatment group showing no spontaneous cartilage repair. Regenerated cartilage was safranin-O positive, contained type II collagen, aggrecan, and human vimentin, and lacked type I collagen, indicating that the hyaline-like neocartilage formed originates from transplanted JCC sheets rather than host-derived cells. This study demonstrates the safety of JCC sheets and stable hyaline cartilage formation with engineered JCC sheets utilizing a sustainable tissue supply. Cost-benefit and scaling issues for sheet fabrication and use support feasibility of this JCC sheet strategy in clinical cartilage repair.
DOI 10.1038/s41536-021-00173-9
PMID 34654830