オカノ　テルオ   OKANO Teruo   岡野　光夫    所属   研究施設 研究施設    職種   特任顧問
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Safety and efficacy of human juvenile chondrocyte-derived cell sheets for osteochondral defect treatment.
掲載誌名	正式名：NPJ Regenerative medicine 略　 称：NPJ Regen Med ISSNコード：20573995/20573995
掲載区分	国外
巻・号・頁	6(1),pp.65
著者・共著者	KONDO Makoto†*, KAMEISHI Sumako, KIM Kyungsook, METZLER Nicolas F, MAAK Travis G, HUTCHINSON Douglas T, WANG Angela A, MAEHARA Miki, GRAINGER David W., OKANO Teruo*
担当区分	最終著者,責任著者
発行年月	2021/10/15
概要	Knee cartilage does not regenerate spontaneously after injury, and a gold standard regenerative treatment algorithm has not been established. This study demonstrates preclinical safety and efficacy of scaffold-free, human juvenile cartilage-derived-chondrocyte (JCC) sheets produced from routine surgical discards using thermo-responsive cultureware. JCCs exhibit stable and high growth potential in vitro over passage 10, supporting possibilities for scale-up to mass production for commercialization. JCC sheets contain highly viable, densely packed cells, show no anchorage-independent cell growth, express mesenchymal surface markers, and lack MHC II expression. In nude rat focal osteochondral defect models, stable neocartilage formation was observed at 4 weeks by JCC sheet transplantation without abnormal tissue growth over 24 weeks in contrast to the nontreatment group showing no spontaneous cartilage repair. Regenerated cartilage was safranin-O positive, contained type II collagen, aggrecan, and human vimentin, and lacked type I collagen, indicating that the hyaline-like neocartilage formed originates from transplanted JCC sheets rather than host-derived cells. This study demonstrates the safety of JCC sheets and stable hyaline cartilage formation with engineered JCC sheets utilizing a sustainable tissue supply. Cost-benefit and scaling issues for sheet fabrication and use support feasibility of this JCC sheet strategy in clinical cartilage repair.
DOI	10.1038/s41536-021-00173-9
PMID	34654830