オカノ　テルオ   OKANO Teruo   岡野　光夫    所属   研究施設 研究施設    職種   特任顧問
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Use of a microchamber for analysis of thermal variation of the gliding phenomenon of single Navicula pavillardii cells.
掲載誌名	正式名：European biophysics journal : EBJ 略　 称：Eur Biophys J ISSNコード：14321017/01757571
掲載区分	国外
巻・号・頁	44(3),pp.113-119
著者・共著者	UMEMURA Kazuo†*, MIYABAYASHI Toru, TAIRA Hisao, SUZUKI Akira, KUMASHIRO Yoshikazu, OKANO Teruo, MAYAMA Shigeki
発行年月	2015/04
概要	In this study, we used a microchamber to observe and analyze the gliding phenomenon of Navicula pavillardii diatom cells at different temperatures. The temperature of the culture medium was varied from 17.0 to 30.0 °C to examine the effect of temperature on diatom movement. Movement of each cell at different temperatures was monitored by use of an inverted optical microscope and continuously recorded as video data, from which the velocities of each cell were calculated, by using dedicated software to perform two-dimensional trajectory analysis. The velocities of the same cell at different temperatures were thereby successfully compared. The results showed that the change in cell velocity was insignificant when the temperature was increased from 17.0 to 25.0 °C. When the temperature was increased from 17.0 to 27.5 °C, non-uniformly disrupted cell movement was observed. When the temperature was further increased to 30.0 °C, cell movement was clearly inhibited. By use of single-cell analysis, the effects of the temperature increases on diatom movement were successfully evaluated. Finally, we characterized the experimental data by performing t tests to evaluate the effects of variations of the movement of individual cells on the data analysis.
DOI	10.1007/s00249-015-1006-0
PMID	25633913