OKANO Teruo    Department   Research Institutes and Facilities, Research Institutes and Facilities    Position
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Mesenchylmal Stem Cell Culture on Poly(N-isopropylacrylamide) Hydrogel with Repeated Thermo-Stimulation.
Journal	Formal name：International journal of molecular sciences Abbreviation：Int J Mol Sci ISSN code：14220067/14220067
Domestic / Foregin	Foregin
Volume, Issue, Page	19(4),pp.1253
Author and coauthor	AKIMOTOAya Mizutani†*, NIITSUErika Hasuike, NAGASEKenichi, OKANOTeruo, KANAZAWAHideko, YOSHIDARyo*
Publication date	2018/04
Summary	We prepared thermoresponsive hydrogels by mixing poly(N-isopropylacrylamide) (PNIPAAm) derivatives as the main chain components, octa-arm polyethylene glycol (PEG) as a crosslinker, and the Arg-Gly-Asp-Ser (RGDS) peptides as cell adhesion units. Human bone marrow-derived mesenchymal stem cells (hbmMSCs) were cultured on the hydrogels. The PNIPAAm gel prepared by the post-crosslinking gelation method was revealed to be cytocompatible and showed temperature-dependent changes in mechanical properties. Repeated changes in the swelling ratio of the PNIPAAm gel affected the shape of the hbmMSCs. With respect to both cytocompatibility and reversibility of changes in mechanical properties, the PNIPAAm gel system could be potentially useful for the analysis of cell mechanobiology.
DOI	10.3390/ijms19041253
PMID	29690540