OKANO Teruo
   Department   Research Institutes and Facilities, Research Institutes and Facilities
   Position  
Article types Original article
Language English
Peer review Peer reviewed
Title Developing palatal bone using human mesenchymal stem cell and stem cells from exfoliated deciduous teeth cell sheets
Journal Formal name：Journal of tissue engineering and regenerative medicine
Abbreviation：J Tissue Eng Regen Med
ISSN code：(1932-7005)1932-6254(Linking)
Domestic / ForeginForegin
Publisher Wiley
Volume, Issue, Page 13(2),pp.319-327
Author and coauthor LEE Jong-Min†, KIM Hyun-Yi, PARK Jin-Sung, LEE Dong-Joon, ZHANG Sushan, GREEN David William, OKANO Teruo, HONG Jeong-Ho, JUNG Han-Sung*
Publication date 2019/02
Summary Cleft palate is one of the most common craniofacial defects in newborn babies. The characteristics of this genetic disease produce soft and hard tissue defects on the lip and maxilla, which cause not only esthetic but also functional problems with speech, eating and breathing. Bone grafts using autologous cancellous bone have been a standard treatment to repair the hard tissue defect in cleft palates. However, such grafts do not fully integrate into host bone and undergo resorption. To overcome engraftment problems, it is common to engineer new tissues with a combination of multipotent cells and biomaterial frameworks. Here, we manufactured cell sheets for bone repair of cleft palates derived from two osteogenic cell sources, human mesenchymal stem cells (hMSC) and stem cells from human exfoliated deciduous teeth (SHED). Cell sheets made from hMSCs and SHEDs gave rise to in vitro calcification, which indicated the osteogenic potential of these cells. The cell sheets of hMSCs and SHEDs expressed the bone-specific osteogenic markers, Osterix (OSX), Osteocalcin (OCN) and Osteopontin (OPN), following insertion into ex vivo-cultured embryonic palatal shelves and in ovo culture. In conclusion, we showed that osteogenic stem cell sheets have mineralization potential and might represent a new alternative to autologous bone transplantation in the reconstruction of cleft palates.
DOI 10.1002/term.2811
PMID 30644640