OKANO Teruo    Department   Research Institutes and Facilities, Research Institutes and Facilities    Position
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Transportation of transplantable cell sheets fabricated with temperature-responsive culture surfaces for regenerative medicine.
Journal	Formal name：Journal of tissue engineering and regenerative medicine Abbreviation：J Tissue Eng Regen Med ISSN code：(1932-6254)1932-6254(Linking)
Domestic / Foregin	Foregin
Volume, Issue, Page	2(4),pp.190-5
Author and coauthor	Nozaki Takayuki†, Yamato Masayuki, Inuma Toshiaki, Nishida Kohji, Okano Teruo*
Publication date	2008/05
Summary	Here we report transportation of cell sheets fabricated on temperature-responsive culture surfaces for regenerative medicine. On the surfaces cells adhere, spread and proliferate at 37 degrees C, but upon temperature reduction below 32 degrees C all the cells are spontaneously detached. When cells on the surfaces are challenged by long distance transportation, maintaining the temperature is critical. Therefore, we developed a portable homothermal container to keep the inner temperature at 36 degrees C for > 30 h without any need for batteries or energy supply. We transported and compared fibroblast sheets cultured on temperature-responsive surfaces in the container, at room temperature in a car, or on ice. After 8 h transportation by car, all cells at room temperature and on ice were detached from the surfaces and some were folded and broken into tiny pieces. On the other hand, fibroblast sheets transported in the container retained their adhesion to the dish surfaces and intact cell sheets were successfully harvested by temperature reduction. During the transportation, cell viability and histology were not impaired. This unique transportation device would be useful for cell sheet-based regenerative medicine utilizing temperature-responsive culture surfaces.
DOI	10.1002/term.80
PMID	18493908