OKANO Teruo    Department   Research Institutes and Facilities, Research Institutes and Facilities    Position
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Engineering liver tissues under the kidney capsule site provides therapeutic effects to hemophilia B mice.
Journal	Formal name：Cell transplantation Abbreviation：Cell Transplant ISSN code：(1555-3892)0963-6897(Linking)
Domestic / Foregin	Foregin
Volume, Issue, Page	19(6),pp.807-13
Author and coauthor	Ohashi Kazuo†*, Tatsumi Kohei, Utoh Rie, Takagi Soichi, Shima Midori, Okano Teruo
Authorship	Last author
Publication date	2010/06
Summary	Recent advances in liver tissue engineering have encouraged further investigation into the evaluation of therapeutic benefits based on animal disease models. In the present study, liver tissues were engineered in coagulation factor IX knockout (FIX-KO) mice, a mouse model of hemophilia B, to determine if the tissue engineering approach would provide therapeutic benefits. Primary hepatocytes were isolated from the liver of wild-type mice and suspended in a mixture of culture medium and extracellular matrix components. The hepatocyte suspension was injected into the space under the bilateral kidney capsules of the FIX-KO mice to engineer liver tissues. The plasma FIX activities (FIX:C) of the untreated FIX-KO mice were undetectable at any time point. In contrast, the liver tissue engineered FIX-KO mice achieved 1.5-2.5% of plasma FIX activities (FIX:C) and this elevated FIX:C level persisted throughout the 90 day experimental period. Significant FIX mRNA expression levels were found in the engineered liver tissues at levels similar to the wild-type livers. The present study demonstrates that liver tissue engineering could provide therapeutic benefits in the treatment of hemophilia B.
DOI	10.3727/096368910X508924
PMID	20573301