オカノ　テルオ   OKANO Teruo   岡野　光夫    所属   研究施設 研究施設    職種   特任顧問
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Irreversible optical clearing of rabbit dermis for autogenic corneal stroma transplantation.
掲載誌名	正式名：Biomaterials 略　 称：Biomaterials ISSNコード：(1878-5905)0142-9612(Linking)
掲載区分	国外
出版社	Elsevier
巻・号・頁	32(28),pp.6764-6772
著者・共著者	Tanaka Yuji†, Shi Dong, Kubota Akira, Takano Yoshimasa, Fuse Nobuo, Yamato Masayuki, Okano Teruo, Nishida Kohji*
発行年月	2011/10
概要	Tissue engineering and transplantation of autogenic grafts have been widely investigated for solving problems on current allograft treatments (i.g., donor shortage and rejection). However, it is difficult to obtain an autogenic corneal stromal replacement that is composed of transparent, tough, and thick collagen constructs by current cell culture-based tissue engineering. Aim of this study is to develop transparent dermis for an autogenic corneal stroma transplantation. This study examined dehydration at 4-8°C and carbodiimide cross-linking on cloudy rabbit dermis (approx. 1.8%-3.8% light transmittance at 550 nm) for dermis optical clearing. Transparency of dehydrated rabbit dermis was founded to be approx. 37.9%-41.4% at 550 nm. Additional cross-linking treatment on dehydrated dermis prevented from swelling and clouding in saline, and improved its transparency to be 56.9% at 550 nm. Rabbit corneal epithelium was found to regenerate on optically cleared dermis in vitro. Furthermore, no abnormal biological response (i.e., inflammation, vascularization, and the barrier defect of epithelia) or no optical functional change on optically cleared dermis was observed during its 4-week autogenic transplantation into rabbit corneal stromal pocket.
DOI	10.1016/j.biomaterials.2011.05.081
PMID	21715003