|
オカノ テルオ
OKANO Teruo
岡野 光夫 所属 研究施設 研究施設 職種 特任顧問 |
|
| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Aqueous chromatographic system for separation of biomolecules using thermoresponsive polymer modified stationary phase. |
| 掲載誌名 | 正式名：Journal of chromatography. A 略 称：J Chromatogr A ISSNコード：(0021-9673)0021-9673(Linking) |
| 掲載区分 | 国外 |
| 出版社 | Elsevier |
| 巻・号・頁 | 1191(1-2),pp.157-161 |
| 著者・共著者 | Kanazawa Hideko†*, Nishikawa Mayumi, Mizutani Aya, Sakamoto Chikako, Morita-Murase Yuko, Nagata Yoshiko, Kikuchi Akihiko, Okano Teruo |
| 発行年月 | 2008/05 |
| 概要 | We have investigated a new method for HPLC using packing materials modified with a functional polymer, such as thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm). PNIPAAm-modified silica exhibits temperature-controlled hydrophilic-hydrophobic surface property changes in aqueous systems. Temperature-responsive chromatography is performed with an aqueous mobile phase without using an organic solvent. We designed ternary copolymers of NIPAAm introduced 2-(dimethyl-amino) ethyl methacrylate (DMAEMA) as a cationic monomer and butyl methacrylate (BMA) as a hydrophobic monomer. A cationic thermoresponsive hydrogel grafted surface would produce an alterable stationary phase with both thermally regulated hydrophobicity and charge density for separation of bioactive compounds. In this study, we achieved successful separation of lysozyme without the loss of bioactivity by temperature-responsive chromatography. The electrostatic and hydrophobic interactions could be modulated simultaneously with the temperature in an aqueous mobile phase, thus the separation system would have potential applications in the separation of biomolecules. |
| DOI | 10.1016/j.chroma.2008.01.056 |
| PMID | 18289554 |