オカノ　テルオ   OKANO Teruo   岡野　光夫    所属   研究施設 研究施設    職種   特任顧問
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Millisecond treatment of cells using microfluidic devices via two-step carrier-medium exchange.
掲載誌名	正式名：Lab on a chip 略　 称：Lab Chip ISSNコード：(1473-0197)1473-0189(Linking)
掲載区分	国外
出版社	The Royal Sciety of Chemistry
巻・号・頁	8(5),pp.772-778
著者・共著者	Yamada Masumi†, Kobayashi Jun, Yamato Masayuki, Seki Minoru, Okano Teruo*
担当区分	最終著者,責任著者
発行年月	2008/05
概要	We present herein a simple but versatile microfluidic system for the treatment of cells with millisecond chemical stimulus, by rapidly exchanging the carrier-medium of cells twice in a microchannel. A technique we refer to as 'hydrodynamic filtration' has been employed for the exchange of medium, in which the virtual width of flow in the microchannel determines the size of filtered cells/particles. The treatment time of cells could be rigidly adjusted by controlling the inlet flow rates and by changing the volume of the stimulating area in the microchannel. In the experiment, two types of microdevices were designed and fabricated, and at first, the ability for carrier-medium exchange was confirmed. As an application of the presented system, we examined the influence of the treatment time of HeLa cells with Triton X-100, a non-ionic surfactant used to solubilize the cellular membrane, on cell viability, varying the average treatment time from 17 to 210 ms. Both quantitative and qualitative analyses were performed to estimate the damage on cell membrane, demonstrating that the cell viability dramatically decreased when the treatment time was longer than approximately 40 ms. The obtained results demonstrated the ability of the presented system to conduct the rapid stimulation of cells, which would be useful for the analysis of biochemical reactions at the cell surface.
DOI	10.1039/b718281c
PMID	18432348