OKANO Teruo    Department   Research Institutes and Facilities, Research Institutes and Facilities    Position
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	In vivo cell tracking by bioluminescence imaging after transplantation of bioengineered cell sheets to the knee joint.
Journal	Formal name：Biomaterials Abbreviation：Biomaterials ISSN code：1878-5905(Electronic)0142-9612(Linking)
Volume, Issue, Page	35(7),pp.2199-206
Author and coauthor	Takaku Yuko†, Murai Kunihiko, Ukai Taku, Ito Satoshi, Kokubo Mami, Satoh Masaaki, Kobayashi Eiji, Yamato Masayuki, Okano Teruo, Takeuchi Mamoru, Mochida Joji, Sato Masato*
Publication date	2014/02
Summary	In our previous studies, we have demonstrated effective regeneration of cartilage through the creation and application of layered cell sheets that combine both chondrocytes and synovial cells. In this study, we were able to demonstrate that cells derived from cell sheets can survive for long periods after transplantation into rat knee joints having osteochondral defects. We established a method for generating cell sheets from firefly luciferase-expressing chondrocytes obtained from transgenic Lewis rats, and carried out allogenic transplantation of these cell sheets into wild-type Lewis rats. We then administered luciferin and monitored the survival of the transplanted cells by using bioluminescence imaging (BLI). Our data showed that the transplanted cells survived and could be detected for more than 21 months, which was longer than expected. Furthermore, the BLI data showed that the transplanted cells remained in the knee joint and did not migrate to other parts of the body, thus confirming the safety of the cell sheets. In this study, we monitored the duration of survival of cell sheets composed of only chondrocytes, only synovial cells, or both chondrocytes and synovial cells, and found that all three types of cell sheets survived for an extended period of time.
DOI	10.1016/j.biomaterials.2013.11.071
Document No.	24360579