オカノ　テルオ   OKANO Teruo   岡野　光夫    所属   研究施設 研究施設    職種   特任顧問
論文種別	総説
言語種別	英語
査読の有無	査読あり
表題	Thermoresponsive thin hydrogel-grafted surfaces for biomedical applications
掲載誌名	正式名：Reactive and Functional Polymers ISSNコード：1381-5148
巻・号・頁	73(7),pp.939-944
著者・共著者	Kobayashi Jun†, Okano Teruo*
担当区分	責任著者
発行年月	2013/07
概要	Thin poly(N-isopropylacrylamide) (PIPAAm) hydrogels were introduced onto biomaterials surfaces for accelerating the kinetics of the swelling and shrinking for PIPAAm hydrogels in response to temperature changes. Thin PIPAAm hydrogels on the biomaterials surfaces exhibit rapid and reversible phase transitions and act as switching sequences to regulate the interaction between the surfaces and biological materials by external temperature-induced changes. By utilizing the temperature-dependent changes of thin PIPAAm hydrogels grafted onto surfaces, our laboratory has pursued unique approaches for developing useful biomedical materials as new types of chromatographic matrices and cell culture surfaces. Aqueous thermoresponsive chromatography systems using PIPAAm-grafted stationary phases enable us to separate biomolecules with high biological activity. Additionally, thermoresponsive cell culture surfaces allow for the recovery of confluent cell monolayers, which have been clinically applied to ophthalmological treatments, dilated cardiomyopathy, esophageal ulcerations, periodontal disease, and cartilage injury. Furthermore, next-generation thermoresponsive cell culture surfaces for large-scale cell cultivation and the capture of specific cells have been considered a key technology for expanding small quantities of stem cells and isolating the resulting differentiated cells for therapeutic use.
DOI	10.1016/j.reactfunctpolym.2013.01.017