SAITO Taiichi
Department Research Institutes and Facilities, Research Institutes and Facilities Position |
|
Article types | Original article |
Language | English |
Peer review | Peer reviewed |
Title | Retinoblastoma protein prevents staurosporine-induced cell death in a retinoblastoma-defective human glioma cell line. |
Journal | Formal name:Pathobiology : journal of immunopathology, molecular and cellular biology Abbreviation:Pathobiology ISSN code:1015-2008(Print)1015-2008(Linking) |
Domestic / Foregin | Foregin |
Volume, Issue, Page | 74(1),pp.22-31 |
Author and coauthor | Yamasaki Fumiyuki, Kajiwara Yoshinori, Hama Seiji, Murakami Taro, Hidaka Toshikazu, Saito Taiichi, Yoshioka Hiroyuki, Sugiyama Kazuhiko, Arita Kazunori, Kurisu Kaoru |
Publication date | 2007/07 |
Summary | OBJECTIVE:To investigate the mechanism of staurosporine-induced glioma cell death and cell cycle arrest using adenovirus-mediated gene transfection, as well as the function of retinoblastoma (Rb) and genetic instability induced by staurosporine.METHODS:Cell cycle regulation, cell death and nuclear abnormalities induced by staurosporine were examined using an adenovirus vector expressing Rb, p16 or p21 genes in human glioma cell lines.RESULTS:The Rb-defective SF-539 cell line was resistant to staurosporine compared with cell lines expressing intact Rb. SF-539 glioma cells exposed to staurosporine became multinucleated and then died. Multinucleation was prevented in SF-539 cells transfected with the Rb gene, thus decreasing the death rate of these cells.CONCLUSIONS:These results imply that enforced Rb expression protects cells from genomic instability induced by staurosporine regardless of its upstream molecular effects. |
DOI | 10.1159/000101048 |
Document No. | 17496430 |