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論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Retinoblastoma protein prevents staurosporine-induced cell death in a retinoblastoma-defective human glioma cell line.
掲載誌名	正式名：Pathobiology : journal of immunopathology, molecular and cellular biology 略　 称：Pathobiology ISSNコード：1015-2008(Print)1015-2008(Linking)
掲載区分	国外
巻・号・頁	74(1),pp.22-31
著者・共著者	Yamasaki Fumiyuki, Kajiwara Yoshinori, Hama Seiji, Murakami Taro, Hidaka Toshikazu, Saito Taiichi, Yoshioka Hiroyuki, Sugiyama Kazuhiko, Arita Kazunori, Kurisu Kaoru
発行年月	2007/07
概要	OBJECTIVE:To investigate the mechanism of staurosporine-induced glioma cell death and cell cycle arrest using adenovirus-mediated gene transfection, as well as the function of retinoblastoma (Rb) and genetic instability induced by staurosporine.METHODS:Cell cycle regulation, cell death and nuclear abnormalities induced by staurosporine were examined using an adenovirus vector expressing Rb, p16 or p21 genes in human glioma cell lines.RESULTS:The Rb-defective SF-539 cell line was resistant to staurosporine compared with cell lines expressing intact Rb. SF-539 glioma cells exposed to staurosporine became multinucleated and then died. Multinucleation was prevented in SF-539 cells transfected with the Rb gene, thus decreasing the death rate of these cells.CONCLUSIONS:These results imply that enforced Rb expression protects cells from genomic instability induced by staurosporine regardless of its upstream molecular effects.
DOI	10.1159/000101048
文献番号	17496430