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和田 圭司
Department Other, Other Position |
|
| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | UnpEL/Usp4 is ubiquitinated by Ro52 and deubiquitinated by itself. |
| Journal | Formal name：Biochemical and biophysical research communications Abbreviation：Biochem Biophys Res Commun ISSN code：0006291X/0006291X |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 342(1),pp.253-8 |
| Author and coauthor | Wada Keiji, Kamitani Tetsu |
| Publication date | 2006/03 |
| Summary | The autoantigen Ro52 is an E3 ubiquitin ligase that can ubiquitinate itself (self-ubiquitination). Recently, we showed that UnpEL/Usp4 is an isopeptidase that can deconjugate ubiquitin from self-ubiquitinated Ro52. Here, we showed that UnpEL is ubiquitinated by Ro52 in cooperation with UbcH5B in vitro. We also showed that UnpEL is ubiquitinated by Ro52 in HEK293T cells. Interestingly, a catalytically inactive UnpEL mutant was strongly ubiquitinated by Ro52 in HEK293T cells. These results suggest that wild-type UnpEL is ubiquitinated by Ro52 and deubiquitinated by itself (self-deubiquitination), while mutant UnpEL is ubiquitinated by Ro52 but not deubiquitinated by itself. In conclusion, Ro52 and UnpEL transregulate each other by ubiquitination and deubiquitination. |
| DOI | 10.1016/j.bbrc.2006.01.144 |
| PMID | 16472766 |