AKIYAMA Yoshikatsu
   Department   Research Institutes and Facilities, Research Institutes and Facilities
   Position   Assistant Professor
Article types Original article
Language English
Peer review Peer reviewed
Title Proliferation of mammalian cells with Chlorococcum littorale algal compounds without serum support.
Journal Formal name:Biotechnology progress
Abbreviation:Biotechnol Prog
ISSN code:15206033/15206033
Domestic / ForeginForegin
Volume, Issue, Page 40(1),pp.e3402
Author and coauthor Ghosh Jayeesha†, Akiyama Yoshikatsu, Haraguchi Yuji, Yamanaka Kumiko, Asahi Toru, Nakao Yoichi, Shimizu Tatsuya*
Authorship 2nd author
Publication date 2023/10
Summary In recent years, serum-free medium for mammalian cell cultivation has attracted a lot of attention, considering the high cost of production and environmental load involved in developing the conventional animal sera. The use of alternative growth-promoting products in mammalian cell cultivation such as extracts from microalgae has proven to be quite beneficial and environmental-friendly. This research aims to cultivate mammalian cells with growth-promoting factors derived from Chlorococcum littorale. We have established a simple extraction using the ultrasonication method and applied the extract in place of serum on mammalian C2C12 cell lines, 3T3 cell lines, and CHO cell lines to compare and analyze the effectiveness of the extract. Cell passage was conducted in a suspended culture condition with the addition of the extract. The results indicate that the extract from microalgae shows a high proliferation rate in all cell lines without fetal bovine serum. Moreover, it is eco-friendly and has huge potential to replace the traditional cell culture system. It could be applied in the fields of regenerative medicine, gene/cell therapies, as well as cultured meat production.
DOI 10.1002/btpr.3402
PMID 37904720