アキヤマ ヨシカツ   AKIYAMA Yoshikatsu
  秋山 義勝
   所属   研究施設 研究施設
   職種   講師
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Proliferation of mammalian cells with Chlorococcum littorale algal compounds without serum support.
掲載誌名 正式名:Biotechnology progress
略  称:Biotechnol Prog
ISSNコード:15206033/15206033
掲載区分国外
巻・号・頁 pp.e3402
著者・共著者 Ghosh Jayeesha†, Akiyama Yoshikatsu, Haraguchi Yuji, Yamanaka Kumiko, Asahi Toru, Nakao Yoichi, Shimizu Tatsuya*
担当区分 2nd著者
発行年月 2023/10
概要 In recent years, serum-free medium for mammalian cell cultivation has attracted a lot of attention, considering the high cost of production and environmental load involved in developing the conventional animal sera. The use of alternative growth-promoting products in mammalian cell cultivation such as extracts from microalgae has proven to be quite beneficial and environmental-friendly. This research aims to cultivate mammalian cells with growth-promoting factors derived from Chlorococcum littorale. We have established a simple extraction using the ultrasonication method and applied the extract in place of serum on mammalian C2C12 cell lines, 3T3 cell lines, and CHO cell lines to compare and analyze the effectiveness of the extract. Cell passage was conducted in a suspended culture condition with the addition of the extract. The results indicate that the extract from microalgae shows a high proliferation rate in all cell lines without fetal bovine serum. Moreover, it is eco-friendly and has huge potential to replace the traditional cell culture system. It could be applied in the fields of regenerative medicine, gene/cell therapies, as well as cultured meat production.
DOI 10.1002/btpr.3402
PMID 37904720