アキヤマ　ヨシカツ   AKIYAMA Yoshikatsu   秋山　義勝    所属   研究施設 研究施設    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
招待の有無	招待あり
表題	Covalent Immobilization of Collagen Type I to a Polydimethylsiloxane Surface for Preventing Cell Detachment by Retaining Collagen Molecules under Uniaxial Cyclic Mechanical Stretching Stress.
掲載誌名	正式名：Biomacromolecules 略　 称：Biomacromolecules ISSNコード：15264602/15257797
掲載区分	国外
巻・号・頁	24(11),pp.5035-5045
著者・共著者	MORI Kazuaki†, KATAOKA Kosuke, AKIYAMA Yoshikatsu*, ASAHI Toru
担当区分	責任著者
発行年月	2023/10/06
概要	Surface modification of polydimethylsiloxane (PDMS) with an extracellular matrix (ECM) is useful for enhancing stable cell attachment. However, few studies have investigated the correlation between the stability of deposited ECM and cell behavior on the PDMS surfaces in external stretched cell culture systems. Herein, covalent collagen type I (Col)-immobilized PDMS surfaces were fabricated using 3-aminopropyl-trimethoxysilane, glutaraldehyde, and Col molecules. The immobilized collagen molecules on the PDMS surface were more stable and uniform than the physisorbed collagen. The cells stably adhered to the Col-immobilized surface and proliferated even under uniaxial cyclic mechanical stretching stress (UnCyMSt), whereas the cells gradually detached from the Col-physisorbed PDMS surface, accompanied by a decrease in the number of deposited collagen molecules. Moreover, the immobilization of collagen molecules enhanced cell alignment under the UnCyMSt. This study reveals that cell adhesion, proliferation, and alignment under the UnCyMSt can be attributed to the retention of collagen molecules on the PDMS surface.
DOI	10.1021/acs.biomac.3c00669
PMID	37800307