AKIYAMA Yoshikatsu
   Department   Research Institutes and Facilities, Research Institutes and Facilities
   Position   Assistant Professor
Article types Original article
Language English
Peer review Peer reviewed
Title Influence of poly(N-isopropylacrylamide) (PIPAAm) graft density on properties of PIPAAm grafted poly(dimethylsiloxane) surfaces and their stability.
Journal Formal name:Heliyon
ISSN code:24058440/24058440
Domestic / ForeginForegin
Volume, Issue, Page 7(3),pp.e06520
Author and coauthor AKIYAMA Yoshikatsu†*
Authorship Lead author,Corresponding author
Publication date 2021/03
Summary A previous report shows that poly(N-isopropylacrylamide) (PIPAAm) gel grafted onto poly(dimethylsiloxane) (PDMS) (PI-PDMS) surfaces with large PIPAAm graft density (Lar-PI-PDMS), is prepared by using electron beam irradiation, demonstrating that applied mechanical stretching affects properties of the Lar-PI-PDMS surface. However, the influence of PIPAAm graft density on the properties of PI-PDMS surfaces and their stability are not understood. To provide insight into these points, the properties of PI-PDMS surfaces with low PIPAAm graft density (Low-PI-PDMS) surfaces with stretched (stretch ratio = 20%) and unstretched states were examined as stretchable temperature-responsive cell culture surface using contact angle measurement and cell attachment/detachment assays, compared to those with Lar-PI-PDMS, as previously reported. Long-term contact angle measurements (61 days) for unstretched Low-PI-PDMS and Lar-PI-PDMS surfaces indicated that the cross-linked structure of the grafted PIPAAm gel suppressed hydrophobic recovery of the basal PDMS surface. The cell attachment assay revealed that the stretched Low-PI-PDMS surface was less cell adhesive than that of the unstretched Low-PI-PDMS surface despite of a larger amount of adsorbed fibronectin (FN). The lower cell adhesiveness was possibly explained by denaturation of adsorbed FN, which was induced by the strong hydrophobic property of the stretched Low-PI-PDMS surface. The cell detachment assay revealed that dual stimuli, low temperature treatment and mechanical shrinking stress applied to the stretched Low-PI-PDMS surface promoted cell detachment compared to a single stimulus, low temperature treatment or mechanical shrinking stress. These results suggested that the PIPAAm gelgrafted PDMS surface was chemically stable and did not suffer from hydrophobic recovery. External mechanical stretching stress not only strongly dehydrated grafted PIPAAm chains, but also denatured the adsorbed FN when the grafted PIPAAm layer
DOI 10.1016/j.heliyon.2021.e06520
PMID 33786400