アキヤマ ヨシカツ   AKIYAMA Yoshikatsu
  秋山 義勝
   所属   研究施設 研究施設
   職種   講師
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Regulation of Protein Binding toward a Ligand on Chromatographic Matrixes by Masking and Forced-Releasing Effects Using Thermoresponsive Polymer
掲載誌名 正式名:Analytical Chemistry
略  称:Anal. Chem.
ISSNコード:0003-2700/1520-6882
掲載区分国外
出版社 American Chemical Society
巻・号・頁 74(16),4160-4166頁
著者・共著者 YOSHIZOKO Kimihiro, AKIYAMA Yoshikatsu, YAMANAKA Hidenori, SHINOHARA Yasuro, HASEGAWA Yukio, Carredano Enrique, KIKUCHI Akihiko, OKANO Teruo
発行年月 2002/07/28
概要 A novel concept of affinity regulation based on masking and forced-releasing effects using a thermoresponsive polymer was elucidated. Affinity chromatographic matrixes were prepared using either poly(glycidyl methacrylate-co-ethyleneglycol dimethacrylate) or poly(glycidyl methacrylate-co-triethyleneglycol dimethacrylate) beads immobilized with ligand molecule, Cibacron Blue F3G-A (CB), together with poly(N-isopropylacrylamide) (PIPAAm), a polymer with a cloud point of 32 °C. Two different lengths of spacer molecules were used for the immobilization of CB while maintaining the PIPAAm size constant. Chromatographic analyses using bovine serum albumin as a model protein showed a clear correlation between spacer length and binding capacity at temperatures lower than the lower critical solution temperature (LCST) of PIPAAm. The binding capacity under the LCST was significantly reduced only when the calculated spacer length was shorter than the mean size of the extended PIPAAm. Furthermore, the adsorbed protein could be desorbed (released) from the matrix surface by lowering the temperature to below the LCST while maintaining other factors such as pH and ion strength. Selective recovery of human albumin from human sera was demonstrated using this newly developed thermoresponsive affinity column.
DOI https://doi.org/10.1021/ac025523z
researchmap用URL https://researchmap.jp/y_akiyama