アキヤマ ヨシカツ
AKIYAMA Yoshikatsu
秋山 義勝 所属 医学研究科 医学研究科 (医学部医学科をご参照ください) 職種 講師 |
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論文種別 | 原著 |
言語種別 | 英語 |
査読の有無 | 査読あり |
表題 | Regulation of Protein Binding toward a Ligand on Chromatographic Matrixes by Masking and Forced-Releasing Effects Using Thermoresponsive Polymer |
掲載誌名 | 正式名:Analytical Chemistry 略 称:Anal. Chem. ISSNコード:0003-2700/1520-6882 |
掲載区分 | 国外 |
出版社 | American Chemical Society |
巻・号・頁 | 74(16),pp.4160-4166 |
著者・共著者 | YOSHIZOKO Kimihiro, AKIYAMA Yoshikatsu, YAMANAKA Hidenori, SHINOHARA Yasuro, HASEGAWA Yukio, Carredano Enrique, KIKUCHI Akihiko, OKANO Teruo |
担当区分 | 2nd著者 |
発行年月 | 2002/07/28 |
概要 | A novel concept of affinity regulation based on masking and forced-releasing effects using a thermoresponsive polymer was elucidated. Affinity chromatographic matrixes were prepared using either poly(glycidyl methacrylate-co-ethyleneglycol dimethacrylate) or poly(glycidyl methacrylate-co-triethyleneglycol dimethacrylate) beads immobilized with ligand molecule, Cibacron Blue F3G-A (CB), together with poly(N-isopropylacrylamide) (PIPAAm), a polymer with a cloud point of 32 °C. Two different lengths of spacer molecules were used for the immobilization of CB while maintaining the PIPAAm size constant. Chromatographic analyses using bovine serum albumin as a model protein showed a clear correlation between spacer length and binding capacity at temperatures lower than the lower critical solution temperature (LCST) of PIPAAm. The binding capacity under the LCST was significantly reduced only when the calculated spacer length was shorter than the mean size of the extended PIPAAm. Furthermore, the adsorbed protein could be desorbed (released) from the matrix surface by lowering the temperature to below the LCST while maintaining other factors such as pH and ion strength. Selective recovery of human albumin from human sera was demonstrated using this newly developed thermoresponsive affinity column. |
DOI | https://doi.org/10.1021/ac025523z |
researchmap用URL | https://researchmap.jp/y_akiyama |