アキヤマ ヨシカツ   AKIYAMA Yoshikatsu
  秋山 義勝
   所属   研究施設 研究施設
   職種   講師
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Temperature-responsive culture surfaces for insect cell sheets to fabricate a bioactuator
掲載誌名 正式名:Advanced robotics : the international journal of the RoboticsSociety of Japan.
略  称:Adv Robot
ISSNコード:01691864
掲載区分国内
出版社 Taylor & Francis
巻・号・頁 33(5),219-231頁
著者・共著者 UESUGI Kaoru†, SAKUMA Yui, AKIYAMA Yoshitake, AKIYAMA Yoshikatsu, IWABUCHI Kikuko, OKANO Teruo, MORISHIMA Keisuke*
発行年月 2019/01
概要 ABSTRACTFor the first time, we have fabricated insect-derived cell sheets by using temperature-responsive culture surfaces having a phase-transition temperature below 25°C. We prepared the temperature-responsive cell culture surfaces (tissue culture polystyrene, TCPS) by grafting a copolymeric gel consisting of hydrophobic N-tert-butylacrylamide (tBAAm) and N-isopropylacrylamide (IPAAm) units. First, to characterize the hydrophilic and hydrophobic properties of the copolymeric gel-grafted surfaces, static water contact angles of each surface were measured at various temperatures. By increasing the amount of tBAAm in the grafted copolymeric gel, the transition temperature of the gel was shifted to lower temperatures. At 25°C, the grafted copolymeric gel was dehydrated, and the insect-derived cells (AeAl2 cells) adhered on all the copolymeric gel-grafted surfaces. At 20°C, AeAl2 cells cannot adhere on the P(IPAAm-1.62tBAAm)-TCPS surface (the initial molar ratio of IPAAm and tBAAm (tBAAm?=?1.62 mol%)) better than on other surfaces (TCPS and tBAAm?=?4.88, 8.17 mol%). These two findings implied that the lower critical solution temperature of the copolymeric gel-grafted-TCPS existed from 20°C to 25°C. The laminin-coated P(IPAAm-1.62tBAAm)-TCPS surface showed temperature-dependent cell attachment and detachment properties, while AeAl2 cells were not detached from the extracellular matrix uncoated P(IPAAm-1.62tBAAm)-TCPS surface. AeAl2 cells and insect muscle cells were harvested as the respective sheets.
DOI 10.1080/01691864.2019.1568908