AKIYAMA Yoshikatsu
   Department   Research Institutes and Facilities, Research Institutes and Facilities
   Position   Assistant Professor
Article types Original article
Language English
Peer review Peer reviewed
Title Thermally Modulated Cationic Copolymer Brush on Monolithic Silica Rods for High-Speed Separation of Acidic Biomolecules
Journal Formal name:ACS Applied Materials & Interfaces
Abbreviation:ACS Appl. Mater. Interfaces
ISSN code:19448244
Volume, Issue, Page 5(4),pp.1442-1452
Author and coauthor Nagase Kenichi†, Kobayashi Jun, Kikuchi Akihiko, Akiyama Yoshikatsu, Kanazawa Hideko, Okano Teruo
Publication date 2013/02
Summary Poly(N-isopropylacrylamide (IPAAm)-co-2-(dimethylamino)ethylmethacrylate(DMAEMA)-co-tert-butylacrylamide(tBAAm)), a thermoresponsive-cationic-copolymer,
brush-grafted monolithic-silica column was prepared throughsurface-initiated atom transfer radical polymerization (ATRP)for effective thermoresponsive anion-exchange chromatography matrices. ATRP-initiator was grafted on monolithic silicarod surfaces by flowing a toluene solution containing ATRP initiator into monolithic silica-rod columns. IPAAm, DMAEMA, and tBAAm monomers and CuCl/CuCl2/Me6TREN, an ATRP catalytic system, were dissolved in 2-propanol, and the reaction solution was pumped into the preprepared initiator modified columns at 25 °C for 16 h. The constructed copolymer-brush structure on monolithic silica-rod surface was confirmed by X-ray photoelectron spectroscopy (XPS), elemental analysis, scanning electron microscopy (SEM) observation, and gel permeation chromatography (GPC) measurement of grafted copolymer. The prepared monolithic silica-rod columns were also characterized by chromatographic analysis. The cationic copolymer brush modified monolithic silica-rod columns were able to separate adenosine nucleotides with a shorter analysis time (4 min) than thermoresponsive copolymer brush-modified silicabead- packed columns, because of the reduced diffusion path length of monolithic supporting materials. These results indicated
that thermoresponsive cationic copolymer brush grafted monolithic silica-rod column prepared by ATRP was a promising tool for analyzing acidic-bioactive compounds with a remarkably short analysis time.
DOI dx.doi.org/10.1021/am302889j