AKIYAMA Yoshikatsu
   Department   Research Institutes and Facilities, Research Institutes and Facilities
   Position   Assistant Professor
Article types Review article
Language English
Peer review Peer reviewed
Title Temperature-Responsive Polymer Modified Surface for Cell Sheet Engineering
Journal Formal name:polymers
ISSN code:2073-4360
Volume, Issue, Page (4),pp.1478-1498
Author and coauthor Tang Zhonglan†, Akiyama Yoshikatsu, Okano Teruo*
Publication date 2012/08
Summary In the past two decades, as a novel approach for tissue engineering, cell sheet
engineering has been proposed by our laboratory. Poly(N-isopropylacrylamide) (PIPAAm),
which is a well-known temperature-responsive polymer, has been grafted on tissue culture
polystyrene (TCPS) surfaces through an electron beam irradiated polymerization. At 37 °C,
where the PIPAAm modified surface is hydrophobic, cells can adhere, spread on the
surface and grow to confluence. By decreasing temperature to 20 °C, since the surface
turns to hydrophilic, cells can detach themselves from the surface spontaneously and form
an intact cell sheet with extracellular matrix. For obtaining a temperature-induced cell
attachment and detachment, it is necessary to immobilize an ultra thin PIPAAm layer on
the TCPS surfaces. This review focuses on the characteristics of PIAPAm modified
surfaces exhibiting these intelligent properties. In addition, PIPAAm modified surfaces
giving a rapid cell-sheet recovery has been further developed on the basis of the
characteristic of the PIPAAm surface. The designs of temperature-responsive polymer
layer have provided an enormous potential to fabricate clinically applicable
regenerative medicine.
DOI 10.3390/polym4031478