アキヤマ　ヨシカツ   AKIYAMA Yoshikatsu   秋山　義勝    所属   研究施設 研究施設    職種   講師
論文種別	総説
言語種別	英語
査読の有無	査読あり
表題	Temperature-Responsive Polymer Modified Surface for Cell Sheet Engineering
掲載誌名	正式名：polymers ISSNコード：2073-4360
巻・号・頁	(4),pp.1478-1498
著者・共著者	Tang Zhonglan†, Akiyama Yoshikatsu, Okano Teruo*
発行年月	2012/08
概要	In the past two decades, as a novel approach for tissue engineering, cell sheet engineering has been proposed by our laboratory. Poly(N-isopropylacrylamide) (PIPAAm), which is a well-known temperature-responsive polymer, has been grafted on tissue culture polystyrene (TCPS) surfaces through an electron beam irradiated polymerization. At 37 °C, where the PIPAAm modified surface is hydrophobic, cells can adhere, spread on the surface and grow to confluence. By decreasing temperature to 20 °C, since the surface turns to hydrophilic, cells can detach themselves from the surface spontaneously and form an intact cell sheet with extracellular matrix. For obtaining a temperature-induced cell attachment and detachment, it is necessary to immobilize an ultra thin PIPAAm layer on the TCPS surfaces. This review focuses on the characteristics of PIAPAm modified surfaces exhibiting these intelligent properties. In addition, PIPAAm modified surfaces giving a rapid cell-sheet recovery has been further developed on the basis of the characteristic of the PIPAAm surface. The designs of temperature-responsive polymer layer have provided an enormous potential to fabricate clinically applicable regenerative medicine.
DOI	10.3390/polym4031478