アキヤマ ヨシカツ   AKIYAMA Yoshikatsu
  秋山 義勝
   所属   研究施設 研究施設
   職種   講師
論文種別 総説
言語種別 英語
査読の有無 査読あり
表題 Temperature-Responsive Polymer Modified Surface for Cell Sheet Engineering
掲載誌名 正式名:polymers
ISSNコード:2073-4360
巻・号・頁 (4),1478-1498頁
著者・共著者 Tang Zhonglan†, Akiyama Yoshikatsu, Okano Teruo*
発行年月 2012/08
概要 In the past two decades, as a novel approach for tissue engineering, cell sheet
engineering has been proposed by our laboratory. Poly(N-isopropylacrylamide) (PIPAAm),
which is a well-known temperature-responsive polymer, has been grafted on tissue culture
polystyrene (TCPS) surfaces through an electron beam irradiated polymerization. At 37 °C,
where the PIPAAm modified surface is hydrophobic, cells can adhere, spread on the
surface and grow to confluence. By decreasing temperature to 20 °C, since the surface
turns to hydrophilic, cells can detach themselves from the surface spontaneously and form
an intact cell sheet with extracellular matrix. For obtaining a temperature-induced cell
attachment and detachment, it is necessary to immobilize an ultra thin PIPAAm layer on
the TCPS surfaces. This review focuses on the characteristics of PIAPAm modified
surfaces exhibiting these intelligent properties. In addition, PIPAAm modified surfaces
giving a rapid cell-sheet recovery has been further developed on the basis of the
characteristic of the PIPAAm surface. The designs of temperature-responsive polymer
layer have provided an enormous potential to fabricate clinically applicable
regenerative medicine.
DOI 10.3390/polym4031478