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出口 敦子
Department School of Medicine, School of Medicine Position Associate Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Lung Tumor Cell Recruitment Assay. |
| Journal | Formal name：Journal of visualized experiments : JoVE Abbreviation：J Vis Exp ISSN code：1940-087X/1940-087X |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | (144),pp.e53172 |
| Author and coauthor | Tomita Takeshi, Ieguchi Katsuaki, Deguchi Atsuko, Takita Morichika, Tsukahara Fujiko, Hiratsuka Sachie, Maru Yoshiro |
| Publication date | 2019/02 |
| Summary | To investigate the molecular mechanisms governing tumor metastasis, various assays using the mouse as a model animal have been proposed. Here, we demonstrate a simple assay to evaluate tumor cell extravasation or micrometastasis. In this assay, tumor cells were injected through the tail vein, and after a short period, the lungs were dissected and digested to count the accumulated labeled tumor cells. This assay skips the initial step of primary tumor invasion into the blood vessel and facilitates the study of events in the distant organ where tumor metastasis occurs. The number of cells injected into the blood vessel can be optimized to observe a limited number of metastases. It has been reported that stromal cells in the distant organ contribute to metastasis. Thus, this assay could be a useful tool to explore potential therapeutic drugs or devices for prevention of tumor metastasis. |
| DOI | 10.3791/53172 |
| PMID | 30882774 |
| URL for researchmap | https://www.jove.com/t/53172/lung-tumor-cell-recruitment-assay |