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稲井 俊太
Department School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine Position Assistant Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Induction of apoptosis in the vestibule of guinea pigs after middle ear inoculation with lipopolysaccharide (LPS). |
| Journal | Formal name：Auris, nasus, larynx Abbreviation：Auris Nasus Larynx ISSN code：03858146/03858146 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 30(4),pp.355-361 |
| Author and coauthor | Watanabe Ken-ichi†, Jinnouchi Ken, Inai Shunta, Yagi Toshiaki |
| Publication date | 2003/12 |
| Summary | OBJECTIVE:During the process of apoptosis, double-stranded DNA is broken into single-stranded DNA (ssDNA) by the action of caspases and caspase activated deoxyribonuclease (CAD). We immunohistochemically examined the apoptotic changes induced by endotoxin in the vestibule of guinea pigs.METHODS:Lipopolysaccharide (LPS) (5 mg/ml, 0.2 ml), a bacterial endotoxin, was transtympanically injected into the middle ear. 48 h after injection of LPS, animals were sacrificed by intracardiac perfusion of fixative. The temporal bones were then removed and immunohistochemically stained for ssDNA, CAD and caspase 3.RESULTS:ssDNA was detected after 48 h in the dark cell area of the LPS group, not in the sensory epithelium. CAD was observed both in the dark cell area and the sensory epithelium. Caspase 3 was also detected both in the dark cell area and the sensory epithelium.CONCLUSION:Our findings suggest that apoptosis is involved in the dysfunction of the vestibule under inflammatory conditions. |
| DOI | 10.1016/s0385-8146(03)00061-0 |
| PMID | 14656560 |