イナイ　シユンタ   稲井　俊太    所属   医学部 医学科（東京女子医科大学病院）    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Expression of caspase-activated deoxyribonuclease (CAD) and caspase 3 (CPP32) in the cochlea of cisplatin (CDDP)-treated guinea pigs.
掲載誌名	正式名：Auris, nasus, larynx 略　 称：Auris Nasus Larynx ISSNコード：03858146/03858146
掲載区分	国外
巻・号・頁	30(3),pp.219-225
著者・共著者	Watanabe Ken-ichi, Inai Shunta, Jinnouchi Ken, Baba Shunkichi, Yagi Toshiaki
担当区分	2nd著者
発行年月	2003/08
概要	Cisplatin, an anti-cancer drug, is known to induce apoptosis. During apoptosis, double-stranded DNA is broken into single-stranded DNA by the action of caspases and caspase activated deoxyribonuclease (CAD). We immunohistochemically examined the cochlea of guinea pigs for signs of the apoptosis after the administration of cisplatin. Cisplatin (10 mg/kg b.w.) was intraperitoneally injected to guinea pigs and 3 days later, the animals were sacrificed by intracardiac perfusion of 4% paraformaldehyde. The temporal bones were then removed and immunohistochemically stained for CAD and caspase 3, using the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labelling method. CAD was observed in the stria vascularis and the spiral ligament. Caspase 3 was also detected in the stria vascularis, the spiral ligament and the supporting cells of the organ of Corti. These findings suggest that apoptosis is involved in the cochlear damage observed in cancer patients treated with cisplatin.
DOI	10.1016/s0385-8146(03)00049-x
PMID	12927282