イナイ　シユンタ   稲井　俊太    所属   医学部 医学科（東京女子医科大学病院）    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Nuclear-factor kappa B (NF-kappa B)-inducible nitric oxide synthase (iNOS/NOS II) pathway damages the stria vascularis in cisplatin-treated mice.
掲載誌名	正式名：Anticancer research 略　 称：Anticancer Res ISSNコード：02507005/02507005
掲載区分	国外
巻・号・頁	22(6C),pp.4081-4085
著者・共著者	Watanabe Ken-Ichi†, Inai Shunta, Jinnouchi Ken, Bada Shunkichi, Hess Alexander, Michel Olaf, Yagi Toshiaki
担当区分	2nd著者
発行年月	2002/11
概要	BACKGROUND:Cisplatin is reported to damage the stria vascularis of the cochlea. Free radicals, especially large amounts of nitric oxide catalyzed by inducible nitric oxide synthase, are considered to have an important role in this toxicity. The induction of inducible nitric oxide synthase is regulated by nuclear-factor kappa B (NF-kappa B). We examined the damage of the stria vascularis by immunohistochemical techniques.MATERIALS AND METHODS:Cisplatin (15 mg/kg b.w.) was injected intraperitoneally into the mice. Three days after the injection, the cochleas were immunohistochemically-stained using specific antibodies for nuclear-factor kappa B (NF-kappa B), inducible nitric oxide synthase (iNOS) or single-stranded DNA.RESULTS:NF-kappa B was expressed in the cisplatin-treated cochlea, especially in the stria vascularis and the spiral ligament. iNOS was also expressed in the stria vascularis and the spiral ligament. Fragments of DNA were observed only in the stria vascularis.CONCLUSION:The large amounts of NO catalyzed by iNOS led to inner ear dysfunction. Our results indicate that apoptosis is triggered by iNOS and that it mediates the ototoxicity induced by cisplatin.
PMID	12553036