Keiko HIROTA    Department   School of Medicine, School of Medicine    Position   Assistant Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	rRNA adenine methylation requires T07A9.8 gene as rram-1 in Caenorhabditis elegans
Journal	Formal name：The Journal of Biochemistry ISSN code：0021924X/17562651
Domestic / Foregin	Domestic
Volume, Issue, Page	163(6),pp.465-474
Author and coauthor	YOKOYAMA Wataru†, HIROTA Keiko†, WAN Huahua, SUMI Naoaki, MIYATA Mai, ARAOI Sho, NOMURA Naoto, KAKO Koichiro, FUKAMIZU Akiyoshi*
Authorship	Lead author
Publication date	2018/06
Summary	RNAs are post-transcriptionally modified in all kingdoms of life. Of these modifications, base methylations are highly conserved in eukaryote ribosomal RNA (rRNA). Recently, rRNA processing protein 8 (Rrp8) and nucleomethylin (NML) were identified as factors of N1-methyladenosine (m1A) modification in yeast 25 S and mammalian 28 S rRNA, respectively. However, m1A modification of rRNA is still poorly understood in Caenorhabditis elegans (C. elegans). Here, using the liquid chromatography/tandem mass spectrometry analysis and RNA immunoprecipitation assay, we have identified that the m1A modification is located around position 674 (A674) of 26 S rRNA in C. elegans. Furthermore, quantitative PCR-based analysis revealed that T07A9.8, a C. elegans homolog of yeast Rrp8 and human NML, is responsible for m1A modification at A674 of 26 S rRNA. This m1A modification site in C. elegans corresponds to those in yeast 25 S rRNA and human 28 S rRNA. Intriguingly, T07A9.8 is not associated with pre-rRNA transcription under normal nutrient conditions. Since the m1A modification of 26 S rRNA requires T07A9.8 in C. elegans, we designated the gene as rRNA adenine methyltransferase-1 (rram-1).
DOI	10.1093/jb/mvy018