ヒロタ　ケイコ   Keiko HIROTA   廣田　恵子    所属   医学部 医学科    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	rRNA adenine methylation requires T07A9.8 gene as rram-1 in Caenorhabditis elegans
掲載誌名	正式名：The Journal of Biochemistry ISSNコード：0021924X/17562651
掲載区分	国内
巻・号・頁	163(6),pp.465-474
著者・共著者	YOKOYAMA Wataru†, HIROTA Keiko†, WAN Huahua, SUMI Naoaki, MIYATA Mai, ARAOI Sho, NOMURA Naoto, KAKO Koichiro, FUKAMIZU Akiyoshi*
担当区分	筆頭著者
発行年月	2018/06
概要	RNAs are post-transcriptionally modified in all kingdoms of life. Of these modifications, base methylations are highly conserved in eukaryote ribosomal RNA (rRNA). Recently, rRNA processing protein 8 (Rrp8) and nucleomethylin (NML) were identified as factors of N1-methyladenosine (m1A) modification in yeast 25 S and mammalian 28 S rRNA, respectively. However, m1A modification of rRNA is still poorly understood in Caenorhabditis elegans (C. elegans). Here, using the liquid chromatography/tandem mass spectrometry analysis and RNA immunoprecipitation assay, we have identified that the m1A modification is located around position 674 (A674) of 26 S rRNA in C. elegans. Furthermore, quantitative PCR-based analysis revealed that T07A9.8, a C. elegans homolog of yeast Rrp8 and human NML, is responsible for m1A modification at A674 of 26 S rRNA. This m1A modification site in C. elegans corresponds to those in yeast 25 S rRNA and human 28 S rRNA. Intriguingly, T07A9.8 is not associated with pre-rRNA transcription under normal nutrient conditions. Since the m1A modification of 26 S rRNA requires T07A9.8 in C. elegans, we designated the gene as rRNA adenine methyltransferase-1 (rram-1).
DOI	10.1093/jb/mvy018