SASAKI Daisuke    Department   Research Institutes and Facilities, Research Institutes and Facilities    Position   Assistant Professor (Fixed Term)
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Simultaneous measurement of contractile force and field potential of dynamically beating human iPS cell-derived cardiac cell sheet-tissue with flexible electronics
Journal	Formal name：Lab on a chip Abbreviation：Lab Chip ISSN code：14730197/14730189
Domestic / Foregin	Foregin
Volume, Issue, Page	21(20),pp.3899-3909
Author and coauthor	OHYA Takashi†, OHTOMO Haruki, KIKUCHI Tetsutaro, SASAKI Daisuke, KAWAMURA Yohei, MATSUURA Katsuhisa, SHIMIZU Tatsuya, FUKUDA Kenjiro*, SOMEYA Takao*, UMEZU Shinjiro*
Publication date	2021/10/12
Summary	Human induced pluripotent stem (iPS) cell-derived cardiomyocytes are used for in vitro pharmacological and pathological studies worldwide. In particular, the functional assessment of cardiac tissues created from iPS cell-derived cardiomyocytes is expected to provide precise prediction of drug effects and thus streamline the process of drug development. However, the current format of electrophysiological and contractile assessment of cardiomyocytes on a rigid substrate is not appropriate for cardiac tissues that beat dynamically. Here, we show a novel simultaneous measurement system for contractile force and extracellular field potential of iPS cell-derived cardiac cell sheet-tissues using 500 nm-thick flexible electronic sheets. It was confirmed that the developed system is applicable for pharmacological studies and assessments of excitation-contraction coupling-related parameters, such as the electro-mechanical window. Our results indicate that flexible electronics with cardiac tissue engineering provide an advanced platform for drug development. This system will contribute to gaining new insight in pharmacological study of human cardiac function.
DOI	10.1039/D1LC00411E