NAKAYAMA Masamichi    Department   Research Institutes and Facilities, Research Institutes and Facilities    Position   Assistant Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Water Stable Nanocoatings of Poly(N-isopropylacrylamide)-based Block Copolymers on Culture Insert Membrane for Temperature-Controlled Cell Adhesion
Journal	Formal name：Journal of materials chemistry. B. Abbreviation：J Mater Chem B ISSN code：2050750X/20507518
Domestic / Foregin	Foregin
Publisher	Royal Society of Chemistry
Volume, Issue, Page	8(34),pp.7812-7821
Author and coauthor	NAKAYAMA Masamichi†*, TOYOSHIMA Yuki, CHINEN Hiroshi, KIKUCHI Akihiko, YAMATO Masayuki, OKANO Teruo
Authorship	Lead author,Corresponding author
Publication date	2020/07/17
Summary	This study demonstrated the spin-coating of functional diblock copolymers to develop smart culture inserts for thermoresponsive cell adhesion/detachment control. One part of the block components, the poly(n-butyl methacrylate) block, strongly supported the water stable surface-immobilization of the thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm) block, regardless of temperature. The chain length of the PNIPAAm blocks was varied to regulate thermal surface functions. Immobilized PNIPAAm concentrations became larger with increasing chain length (1.0–1.6 μg cm−2) and the thicknesses of individual layers were relatively comparable at 10-odd nanometers. A nanothin coating scarcely inhibited the permeability of the original porous membrane. When human fibroblasts were cultured on each surface at 37 °C, the efficiencies of cell adhesion and proliferation decreased with longer PNIPAAm chains. Meanwhile, by reducing the temperature to 20 °C, longer PNIPAAm chains promoted cell detachment owing to the significant thermoresponsive alteration of cell-surface affinity. Consequently, we successfully produced a favorable cell sheet by choosing an appropriate PNIPAAm length for block copolymers.
DOI	10.1039/D0TB01113D