NAKAYAMA Masamichi    Department   Graduate School of Medical Science, Graduate School of Medical Science    Position   Assistant Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Water Stable Nanocoatings of Poly(N-isopropylacrylamide)-based Block Copolymers on Culture Insert Membrane for Temperature-Controlled Cell Adhesion
Journal	Formal name：Journal of materials chemistry. B. Abbreviation：J Mater Chem B ISSN code：2050750X/20507518
Domestic / Foregin	Foregin
Publisher	Royal Society of Chemistry
Volume, Issue, Page	8(34),pp.7812-7821
Author and coauthor	NAKAYAMA Masamichi†*, TOYOSHIMA Yuki, CHINEN Hiroshi, KIKUCHI Akihiko, YAMATO Masayuki, OKANO Teruo
Authorship	Lead author,Corresponding author
Publication date	2020/07/17
Summary	This study demonstrated the spin-coating of functional diblock copolymers to develop smart culture inserts for thermoresponsive cell adhesion/detachment control. One part of the block components, the poly(n-butyl methacrylate) block, strongly supported the water stable surface-immobilization of the thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm) block, regardless of temperature. The chain length of the PNIPAAm blocks was varied to regulate thermal surface functions. Immobilized PNIPAAm concentrations became larger with increasing chain length (1.0–1.6 μg cm−2) and the thicknesses of individual layers were relatively comparable at 10-odd nanometers. A nanothin coating scarcely inhibited the permeability of the original porous membrane. When human fibroblasts were cultured on each surface at 37 °C, the efficiencies of cell adhesion and proliferation decreased with longer PNIPAAm chains. Meanwhile, by reducing the temperature to 20 °C, longer PNIPAAm chains promoted cell detachment owing to the significant thermoresponsive alteration of cell-surface affinity. Consequently, we successfully produced a favorable cell sheet by choosing an appropriate PNIPAAm length for block copolymers.
DOI	10.1039/D0TB01113D