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Sekine Hidekazu
Department Research Institutes and Facilities, Research Institutes and Facilities Position Associate Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels. |
| Journal | Formal name：Scientific reports Abbreviation：Sci Rep ISSN code：20452322/20452322 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 12(1),pp.21698 |
| Author and coauthor | INOUE NAGAHARA Ayumi†, HOMMA Jun, RYU Bikei, SEKINE Hidekazu*, HIGASHI Yuhei, SHIMIZU Tatsuya, KAWAMATA Takakazu |
| Authorship | Corresponding author |
| Publication date | 2022/12/15 |
| Summary | This study evaluated whether cell sheets containing a network of lymphatic endothelial cells (LECs) promoted lymphangiogenesis after transplantation in vivo. Cell sheets with a LEC network were constructed by co-culturing LECs and adipose-derived stem cells (ASCs) on temperature-responsive culture dishes. A cell ratio of 3:2 (vs. 1:4) generated networks with more branches and longer branch lengths. LEC-derived lymphatic vessels were observed 2 weeks after transplantation of a three-layered cell sheet construct onto rat gluteal muscle. Lymphatic vessel number, diameter and depth were greatest for a construct comprising two ASC sheets stacked on a LEC/ASC (3:2 ratio) sheet. Transplantation of this construct in a rat model of femoral lymphangiectomy led to the formation of functional lymphatic vessels containing both transplanted and host LECs. Further development of this technique may lead to a new method of promoting lymphangiogenesis. |
| DOI | 10.1038/s41598-022-26041-0 |
| PMID | 36522421 |