Sekine Hidekazu    Department   Research Institutes and Facilities, Research Institutes and Facilities    Position   Associate Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	External pressure dynamics promote kidney viability and perfusate filtration during ex vivo kidney perfusion
Journal	Formal name：Scientific Reports Abbreviation：Sci Rep ISSN code：2045-2322
Domestic / Foregin	Foregin
Publisher	Spuringer Nature
Volume, Issue, Page	12(1),pp.21564
Author and coauthor	HIGASHI Yuhei†, HOMMA Jun, SEKINE Hidekazu*, UAGO Hiroki, KOBAYASHI Eiji, SHIMIZU Tatsuya
Authorship	Corresponding author
Publication date	2022/12/13
Summary	Normothermic machine perfusion (NMP) has not yet been established as a technique for preserving organs for a day. A key contributing factor to the same is that the perfusing solutions cannot circulate continuously and evenly in the organs. Here, we conceived a method of applying intermittent air pressure from outside the organ to assist its circulatory distribution during perfusion. We used a perfusion culture system while applying external pressure to culture rat kidneys and compared the circulatory distribution in the kidneys, changes in tissue morphology due to injury, and perfusate filtration. The intermittent pressurization (IMP) (-) group showed markedly poorer circulation on the upper side compared with that in the lower side, alongside histological damage. On the other hand, the IMP (+) group showed improved circulation in the upper side and had lesser histological damage. Furthermore, the IMP (+) group maintained the ability to filter perfusate for 24 h. In transplantation medicine and regenerative medicine research, this method has the potential to contribute to more efficient organ preservation and more functional tissue regeneration in the future.
DOI	10.1038/s41598-022-26147-5