Sekine Hidekazu    Department   Research Institutes and Facilities, Research Institutes and Facilities    Position   Associate Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Microfluidic vascular-bed devices for vascularized 3D tissue engineering: tissue engineering on a chip.
Journal	Formal name：Biomedical microdevices Abbreviation：Biomed Microdevices ISSN code：15728781/13872176
Domestic / Foregin	Foregin
Publisher	Springer
Volume, Issue, Page	22(1),pp.9
Author and coauthor	TAKEHARA Hiroaki†*, SAKAGUCHI Katsuhisa, SEKINE Hidekazu, OKANO Teruo, SHIMIZU Tatsuya*
Publication date	2019/12/20
Summary	In this report, we describe a microfluidic vascular-bed (micro-VB) device providing a platform for 3D tissue engineering with vascular network formation. The micro-VB device allows functional connections between endothelial capillaries of heterogeneous sections (5-100 μm in diameter) and artificial plastic tubes or reservoirs (1-10 mm in diameter). Moreover, the micro-VB device can be installed in a standard 100 mm-diameter Petri dish. Endothelial networks in 3D engineered tissues were obtained by cellular self-assembly on the device, after co-culturing of human umbilical vein endothelial cells (HUVECs) and normal human dermal fibroblasts (NHDFs) in fibrin gel. Endothelial capillary connection between vascularized tissues and microfluidic channels, mimicking arteries and veins, was confirmed by perfusion of fluorescent microspheres. The micro-VB devices were compatible with the use of commercially available culture dishes and did not require the involvement of additional equipment. Thus, these micro-VB devices are expected to substantially improve the routine application of 3D tissue engineering to regenerative medicine.
DOI	10.1007/s10544-019-0461-2
PMID	31863202