田邊 賢司
Department Research Institutes and Facilities, Research Institutes and Facilities Position Associate Professor |
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Article types | Original article |
Language | English |
Peer review | Peer reviewed |
Title | Dynamin 2 associates with microtubules at mitosis and regulates cell cycle progression. |
Journal | Formal name:Cell Structure and Function |
Volume, Issue, Page | 36,pp.145-154 |
Author and coauthor | Ishida, Nobuhisa Nakamura, Yuichi Tanabe, Kenj i Li, Shun-Ai Takei, Kohji |
Authorship | Lead author,Corresponding author |
Publication date | 2011 |
Summary | Dynamin, a ~100 kDa large GTPase, is known as a key player for membrane traffic. Recent evidence shows that dynamin also regulates the dynamic instability of microtubules by a mechanism independent of membrane traffic. As microtubules are highly dynamic during mitosis, we investigated whether the regulation of microtubules by dynamin is essential for cell cycle progression. Dynamin 2 intensely localized at the mitotic spindle, and the localization depended on its proline-rich domain (PRD), which is required for microtubule association. The deletion of PRD resulted in the impairment of cytokinesis, whereby the mutant had less effect on endocytosis. Interestingly, dominant-negative dynamin (K44A), which blocks membrane traffic but has no effect on microtubules, also blocked cytokinesis. On the other hand, the deletion of the middle domain, which binds to γ-tubulin, impaired the entry into mitosis. As both deletion mutants had no significant effect on endocytosis, dynamin 2 may participate in cell cycle progression by regulating the microtubules. These data suggest that dynamin may play a key role for cell cycle progression by twodistinct pathways, membrane traffic and cytoskeleton. |