田邊 賢司
Department Research Institutes and Facilities, Research Institutes and Facilities Position Associate Professor |
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Article types | Original article |
Language | English |
Peer review | Peer reviewed |
Title | Localization of SMAP2 to the TGN and its function in the regulation of TGN protein transport. |
Journal | Formal name:Cell Structure and Function |
Volume, Issue, Page | 36,pp.83-95 |
Author and coauthor | Funaki, Tomo Kon, Shunsuke Ronn, Roger E Henmi, Yuji Kobayashi, Yuka Watanabe, Toshio Nakayama, Keiko Tanabe, Kenji Satake, Masanobu |
Publication date | 2011 |
Summary | SMAP2 is an Arf GTPase-activating protein that is located and functions on early endosome membranes. In the present study, the trans-Golgi network (TGN) was verified as an additional site of SMAP2 localization based on its co-localization with various TGN-marker proteins. Mutation of specific stretches of basic amino acid residues abolished the TGN-localization of SMAP2. Over-expression of wild-type SMAP2, but not of the mutated SMAP2, inhibited the transport of vesicular stomatitis virus-G protein from the TGN to the plasma membrane. In contrast, this transport was enhanced in SMAP2 (-/-) cells characterized by increased levels of the activated form of Arf. SMAP2 therefore belongs to an ArfGAP subtype that resides on the TGN and functions as a negative regulator of vesicle budding from the organelle. |