タナベ ケンジ
  田邊 賢司
   所属   研究施設 研究施設
   職種   准教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Localization of SMAP2 to the TGN and its function in the regulation of TGN protein transport.
掲載誌名 正式名:Cell Structure and Function
巻・号・頁 36,83-95頁
著者・共著者 Funaki, Tomo Kon, Shunsuke Ronn, Roger E Henmi, Yuji Kobayashi, Yuka Watanabe, Toshio Nakayama, Keiko Tanabe, Kenji Satake, Masanobu
発行年月 2011
概要 SMAP2 is an Arf GTPase-activating protein that is located and functions on early endosome membranes. In the present study, the trans-Golgi network (TGN) was verified as an additional site of SMAP2 localization based on its co-localization with various TGN-marker proteins. Mutation of specific stretches of basic amino acid residues abolished the TGN-localization of SMAP2. Over-expression of wild-type SMAP2, but not of the mutated SMAP2, inhibited the transport of vesicular stomatitis virus-G protein from the TGN to the plasma membrane. In contrast, this transport was enhanced in SMAP2 (-/-) cells characterized by increased levels of the activated form of Arf. SMAP2 therefore belongs to an ArfGAP subtype that resides on the TGN and functions as a negative regulator of vesicle budding from the organelle.