田邊　賢司    Department   Research Institutes and Facilities, Research Institutes and Facilities    Position   Associate Professor
Article types	Review article
Language	English
Peer review	Peer reviewed
Title	A SMAP gene family encoding ARF GTPase-activating proteins and its implication in membrane trafficking.
Journal	Formal name：Methods in enzymology
Volume, Issue, Page	438,pp.155-170
Author and coauthor	Tanabe, Kenji Kon, Shunsuke Ichijo, Nobuyuki Funaki, Tomo Natsume, Waka Watanabe, Toshio Satake, Masanobu
Authorship	Lead author
Publication date	2008
Summary	SMAP1 and SMAP2 proteins constitute a subfamily of the Arf-specific GTPase-activating proteins. Both SMAP proteins bind to clathrin heavy chains and are involved in the trafficking of clathrin-coated vesicles. In cells, SMAP1 regulates Arf6-dependent endocytosis of transferrin receptors from the coated pits of the plasma membrane, whereas SMAP2 regulates Arf1-dependent retrograde transport of TGN38 from the early endosome to the trans-Golgi network. The common and distinct features of SMAP1 and SMAP2 activity provide a valuable opportunity to examine the differential regulation of membrane trafficking by these two proteins. In this chapter, we describe several basic experimental procedures that have been used to study the regulation of membrane trafficking using SMAP proteins, including a GAP assay as well as procedures to study the transport of transferrin receptors and TGN38. In addition, a yeast two-hybrid system is described because of its utility in identifying novel molecules that interact with SMAP.