タナベ ケンジ
  田邊 賢司
   所属   研究施設 研究施設
   職種   准教授
論文種別 総説
言語種別 英語
査読の有無 査読あり
表題 A SMAP gene family encoding ARF GTPase-activating proteins and its implication in membrane trafficking.
掲載誌名 正式名:Methods in enzymology
巻・号・頁 438,155-170頁
著者・共著者 Tanabe, Kenji Kon, Shunsuke Ichijo, Nobuyuki Funaki, Tomo Natsume, Waka Watanabe, Toshio Satake, Masanobu
発行年月 2008
概要 SMAP1 and SMAP2 proteins constitute a subfamily of the Arf-specific GTPase-activating proteins. Both SMAP proteins bind to clathrin heavy chains and are involved in the trafficking of clathrin-coated vesicles. In cells, SMAP1 regulates Arf6-dependent endocytosis of transferrin receptors from the coated pits of the plasma membrane, whereas SMAP2 regulates Arf1-dependent retrograde transport of TGN38 from the early endosome to the trans-Golgi network. The common and distinct features of SMAP1 and SMAP2 activity provide a valuable opportunity to examine the differential regulation of membrane trafficking by these two proteins. In this chapter, we describe several basic experimental procedures that have been used to study the regulation of membrane trafficking using SMAP proteins, including a GAP assay as well as procedures to study the transport of transferrin receptors and TGN38. In addition, a yeast two-hybrid system is described because of its utility in identifying novel molecules that interact with SMAP.