タナベ ケンジ
  田邊 賢司
   所属   研究施設 研究施設
   職種   准教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Disruption of microtubule network rescues aberrant actin comets in dynamin2-depleted cells.
掲載誌名 正式名:PLoS ONE
巻・号・頁 6,e28603頁
著者・共著者 Henmi, Yuji Tanabe, Kenji Takei, Kohji
発行年月 2011
概要 A large GTPase dynamin, which is required for endocytic vesicle formation, regulates the actin cytoskeleton through its interaction with cortactin. Dynamin2 mutants impair the formation of actin comets, which are induced by Listeria monocytogenes or phosphatidylinositol-4-phosphate 5-kinase. However, the role of dynamin2 in the regulation of the actin comet is still unclear. Here we show that aberrant actin comets in dynamin2-depleted cells were rescued by disrupting of microtubule networks. Depletion of dynamin2, but not cortactin, significantly reduced the length and the speed of actin comets induced by Listeria. This implies that dynamin2 may regulate the actin comet in a cortactin-independent manner. As dynamin regulates microtubules, we investigated whether perturbation of microtubules would rescue actin comet formation in dynamin2-depleted cells. Treatment with taxol or colchicine created a microtubule-free space in the cytoplasm, and made no difference between controland dynamin2 siRNA cells. This suggests that the alteration of microtubules by dynamin2 depletion reduced the length and the speed of the actin comet.