サカグチ　カツヒサ   坂口　勝久    所属   研究施設 研究施設    職種   非常勤講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Optical coherence microscopy of living cells and bioengineered tissue dynamics in high-resolution cross-section.
掲載誌名	正式名：Journal of biomedical materials research. Part B, Applied biomaterials 略　 称：J Biomed Mater Res B Appl Biomater ISSNコード：(1552-4981)1552-4973(Linking)
掲載区分	国外
巻・号・頁	105(3),pp.481-488
著者・共著者	Hasegawa Akiyuki†, Haraguchi Yuji, Oikaze Hirotoshi, Kabetani Yasuhiro, Sakaguchi Katsuhisa, Shimizu Tatsuya*
発行年月	2017/04
概要	Optical coherence tomography (OCT) is a valuable tool in the cross-sectional observation/analysis of three-dimensional (3-D) biological tissues, and that histological observation is important clinically. However, the resolution of the technology is approximately 10-20 μm. In this study, optical coherence microscopy (OCM), a tomographic system combining OCT technology with a microscopic technique, was constructed for observing cells individually with a resolution at the submicrometer level. Cells and 3-D tissues fabricated by cell sheet technology were observed by OCM. Importantly, the cell nuclei and cytoplasm could be clearly distinguished, and the time-dependent dynamics of cell-sheet tissues could be observed in detail. Additionally, the 3-D migration of cells in the bioengineered tissue was also detected using OCM and metal-labeled cells. Bovine aortic endothelial cells, but not NIH3T3 murine embryonic skin fibroblasts, actively migrated within the 3-D tissues. This study showed that the OCM system would be a valuable tool in the fields of cell biology, tissue engineering, and regenerative medicine. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 481-488, 2017.
DOI	10.1002/jbm.b.33566
PMID	26545952