井口 成一
     School of Medicine School of Medicine(Tokyo Women's Medical University Hospital)
    
Article types Review article
Language English
Peer review Peer reviewed
Invitation Invited paper
Title Translational applications of diagnostics of infectious diseases using infectomics approaches in clinical settings.
Journal Formal name:Journal of Bacteriology and Mycology Open Access
Abbreviation:J Bacteriol Mycol Open Access
Domestic / ForeginForegin
Volume, Issue, Page pp.00071.
Author & co-author Yoshida Atsushi, Iguchi Shigekazu, Uzawa Yutaka, Kikuchi Ken*
Publication date 2016
Summary Modern molecular and biochemical technologies like polymerase chain reaction (PCR), matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), or microarray, evolved the diagnostic strategies of infectious diseases and changed the routine workflow in clinical microbiology laboratories. However, we still cannot identify causative organisms of many infectious diseases, like febrile neutropenia, sepsis, pneumonia, systemic mycoses and culture-negative endocarditis, in critically ill patients even though using such molecular techniques, and consequently we use antimicrobials or antifungals empirically for these cases without any evidence of pathogens specified. Now we need to find out the alternative way for diagnostics of infectious diseases. In the post-genomics era, undiagnosed infectious diseases would be analyzed by comprehensive data mining and hierarchical algorithm obtained from perspective infectome that analyzes host-pathogen-microbiome interactions. Accordingly we require a new translational application in clinical settings from this infectomics. Detecting pathogen-specific or infected host-derived volatile organic compounds is one of the good answers because it is non-invasive, easily performed, rapid, inexpensive, and available for point-of-care testing for diagnosis of infectious diseases. This mini-review focuses unmet needs of diagnostics of infectious diseases in clinical settings and impact of alternative diagnostic ways using infectomics approaches and their clinical implications.
DOI 10.15406/jbmoa.2016.03.00071