井口 成一
   Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine
   Position  
Article types Original article
Language English
Peer review Peer reviewed
Title Potential Impact of Rapid Blood Culture Testing for Gram-Positive Bacteremia in Japan with the Verigene Gram-Positive Blood Culture Test.
Journal Formal name:The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale
Abbreviation:Can J Infect Dis Med Microbiol
ISSN code:(1712-9532)1712-9532(Linking)
Domestic / ForeginForegin
Volume, Issue, Page 2017,4896791頁
Author and coauthor Kikuchi Ken, Matsuda Mari, Iguchi Shigekazu, Mizutani Tomonori, Hiramatsu Keiichi, Tega-Ishii Michiru, Sansaka Kaori, Negishi Kenta, Shimada Kimie, Umemura Jun, Notake Shigeyuki, Yanagisawa Hideji, Takahashi Hiroshi, Yabusaki Reiko, Araoka Hideki, Yoneyama Akiko
Publication date 2017/02
Summary Background. Early detection of Gram-positive bacteremia and timely appropriate antimicrobial therapy are required for decreasing patient mortality. The purpose of our study was to evaluate the performance of the Verigene Gram-positive blood culture assay (BC-GP) in two special healthcare settings and determine the potential impact of rapid blood culture testing for Gram-positive bacteremia within the Japanese healthcare delivery system. Furthermore, the study included simulated blood cultures, which included a library of well-characterized methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE) isolates reflecting different geographical regions in Japan. Methods. A total 347 BC-GP assays were performed on clinical and simulated blood cultures. BC-GP results were compared to results obtained by reference methods for genus/species identification and detection of resistance genes using molecular and MALDI-TOF MS methodologies. Results. For identification and detection of resistance genes at two clinical sites and simulated blood cultures, overall concordance of BC-GP with reference methods was 327/347 (94%). The time for identification and antimicrobial resistance detection by BC-GP was significantly shorter compared to routine testing especially at the cardiology hospital, which does not offer clinical microbiology services on weekends and holidays. Conclusion. BC-GP generated accurate identification and detection of resistance markers compared with routine laboratory methods for Gram-positive organisms in specialized clinical settings providing more rapid results than current routine testing.
DOI 10.1155/2017/4896791
PMID 28316631