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井口 成一
Department School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine Position |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Potential Impact of Rapid Blood Culture Testing for Gram-Positive Bacteremia in Japan with the Verigene Gram-Positive Blood Culture Test. |
| Journal | Formal name：The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale Abbreviation：Can J Infect Dis Med Microbiol ISSN code：(1712-9532)1712-9532(Linking) |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 2017,pp.4896791 |
| Author and coauthor | Kikuchi Ken, Matsuda Mari, Iguchi Shigekazu, Mizutani Tomonori, Hiramatsu Keiichi, Tega-Ishii Michiru, Sansaka Kaori, Negishi Kenta, Shimada Kimie, Umemura Jun, Notake Shigeyuki, Yanagisawa Hideji, Takahashi Hiroshi, Yabusaki Reiko, Araoka Hideki, Yoneyama Akiko |
| Publication date | 2017/02 |
| Summary | Background. Early detection of Gram-positive bacteremia and timely appropriate antimicrobial therapy are required for decreasing patient mortality. The purpose of our study was to evaluate the performance of the Verigene Gram-positive blood culture assay (BC-GP) in two special healthcare settings and determine the potential impact of rapid blood culture testing for Gram-positive bacteremia within the Japanese healthcare delivery system. Furthermore, the study included simulated blood cultures, which included a library of well-characterized methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE) isolates reflecting different geographical regions in Japan. Methods. A total 347 BC-GP assays were performed on clinical and simulated blood cultures. BC-GP results were compared to results obtained by reference methods for genus/species identification and detection of resistance genes using molecular and MALDI-TOF MS methodologies. Results. For identification and detection of resistance genes at two clinical sites and simulated blood cultures, overall concordance of BC-GP with reference methods was 327/347 (94%). The time for identification and antimicrobial resistance detection by BC-GP was significantly shorter compared to routine testing especially at the cardiology hospital, which does not offer clinical microbiology services on weekends and holidays. Conclusion. BC-GP generated accurate identification and detection of resistance markers compared with routine laboratory methods for Gram-positive organisms in specialized clinical settings providing more rapid results than current routine testing. |
| DOI | 10.1155/2017/4896791 |
| PMID | 28316631 |