ミドリカワ ミツハル
  緑川 光春
   所属   医学部 医学科
   職種   講師
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Single secretory granules of live cells recruit syntaxin-1 and synaptosomal associated protein 25 (SNAP-25) in large copy numbers.
掲載誌名 正式名:Proceedings of the National Academy of Sciences of the United States of America
略  称:Proc Natl Acad Sci U S A
ISSNコード:(1091-6490)0027-8424(Linking)
掲載区分国外
巻・号・頁 107(48),20810-20815頁
著者・共著者 Knowles M K, Barg S, Wan L, Midorikawa M, Chen X, Almers Wolfhard
発行年月 2010/11
概要 Before secretory vesicles undergo exocytosis, they must recruit the proteins syntaxin-1 and synaptosomal associated protein 25 (SNAP-25) in the plasma membrane. GFP-labeled versions of both proteins cluster at sites where secretory granules have docked. Single-particle tracking shows that minority populations of both molecules are strongly hindered in their mobility, consistent with their confinement in nanodomains. We measured the fluorescence of granule-associated clusters, the fluorescence of single molecules, and the numbers of unlabeled syntaxin-1 and SNAP-25 molecules per cell. There was a more than 10-fold excess of SNAP-25 over syntaxin-1. Fifty to seventy copies each of syntaxin-1 and SNAP-25 molecules were associated with a single docked granule, many more than have been reported to be required for fusion.
DOI 10.1073/pnas.1014840107
PMID 21076040