ミドリカワ ミツハル
  緑川 光春
   所属   医学部 医学科
   職種   講師
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Syntaxin clusters assemble reversibly at sites of secretory granules in live cells.
掲載誌名 正式名:Proceedings of the National Academy of Sciences of the United States of America
略  称:Proc Natl Acad Sci U S A
ISSNコード:(1091-6490)0027-8424(Linking)
掲載区分国外
巻・号・頁 107(48),20804-20809頁
著者・共著者 Barg S, Knowles M K, Chen X, Midorikawa M, Almers Wolfhard
発行年月 2010/11
概要 Syntaxin resides in the plasma membrane, where it helps to catalyze membrane fusion during exocytosis. The protein also forms clusters in cell-free and granule-free plasma-membrane sheets. We imaged the interaction between syntaxin and single secretory granules by two-color total internal reflection microscopy in PC12 cells. Syntaxin-GFP assembled in clusters at sites where single granules had docked at the plasma membrane. Clusters were intermittently present at granule sites, as syntaxin molecules assembled and disassembled in a coordinated fashion. Recruitment to granules required the N-terminal domain of syntaxin, but not the entry of syntaxin into SNARE complexes. Clusters facilitated exocytosis and disassembled once exocytosis was complete. Syntaxin cluster formation defines an intermediate step in exocytosis.
DOI 10.1073/pnas.1014823107
PMID 21076041