ミドリカワ ミツハル
  緑川 光春
   所属   医学部 医学科
   職種   講師
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Imaging Exocytosis of Single Synaptic Vesicles at a Fast CNS Presynaptic Terminal.
掲載誌名 正式名:Neuron
略  称:Neuron
ISSNコード:(1097-4199)0896-6273(Linking)
掲載区分国外
巻・号・頁 88(3),492-498頁
著者・共著者 Midorikawa Mitsuharu, Sakaba Takeshi
発行年月 2015/11
概要 Synaptic vesicles are tethered to the active zone where they are docked/primed so that they can fuse rapidly upon Ca(2+) influx. To directly study these steps at a CNS presynaptic terminal, we used total internal reflection fluorescence (TIRF) microscopy at the live isolated calyx of Held terminal and measured the movements of single synaptic vesicle just beneath the plasma membrane. Only a subset of vesicles within the TIRF field underwent exocytosis. Following exocytosis, new vesicles (newcomers) approached the membrane and refilled the release sites slowly with a time constant of several seconds. Uniform elevation of the intracellular Ca(2+) using flash photolysis elicited an exocytotic burst followed by the sustained component, representing release of the readily releasable vesicles and vesicle replenishment, respectively. Surprisingly, newcomers were not released within a second of high Ca(2+). Instead, already-tethered vesicles became release-ready and mediated the replenishment. Our results reveal an important feature of conventional synapses.
DOI 10.1016/j.neuron.2015.09.047
PMID 26539890